[1]吕慧成,李斯琴,周荣兴,等.绿色荧光蛋白体外转染兔骨髓基质干细胞[J].内蒙古医科大学学报,2013,35(02):96-101.
 LUHui-cheng,LI Si-qin,ZHOU Rong-xing.GREENFLUORESCENTPROTEINVITROTRANSFECTION OFRABBITBONEMARROWSTROMALSTEMCELLS[J].Acta Academiae Medicinae Neimongol,2013,35(02):96-101.
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绿色荧光蛋白体外转染兔骨髓基质干细胞()
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《内蒙古医科大学学报》[ISSN:1004-2113/CN:15-1129/R]

卷:
第35卷
期数:
2013年02期
页码:
96-101
栏目:
论著
出版日期:
2013-02-28

文章信息/Info

Title:
GREENFLUORESCENTPROTEINVITROTRANSFECTION OFRABBITBONEMARROWSTROMALSTEMCELLS
作者:
吕慧成 12 李斯琴 3 周荣兴 1 李晓东 1 郭军 1 贾海生 1 吴一民 1
1. 重庆医科大学重庆市;2. 内蒙古医科大学第二附属医院创伤二科;3. 内蒙古人民医院超声科
Author(s):
LUHui-chengLI Si-qinZHOU Rong-xing
Two Department of Trauma,The SecondAffiliatedHospital,Inner MongoliaMedical University,Hohhot 010030China
关键词:
骨髓基质干细胞GFP基因转染骨组织工程
Keywords:
bone marrowmesenchymal stemcellsgreenfluorescent proteingene transfection osseous tissueengineering
分类号:
R392.4
摘要:
目的:观察以质粒为载体,经脂质体介导的绿色荧光蛋白(greenfluorescent protein,GFP)转染的骨髓 间充质干细胞体外表达情况。 方法:以质粒病毒为载体,PT67细胞为包装细胞,介导绿色荧光蛋白转染新西兰 大白兔,在相差显微镜和荧光显微镜下观察,流式细胞仪检测GFP的表达效率。 结果:骨髓基质干细胞体外培 养后贴壁、集落生长,连续传代培养生长良好。 培养2wk后骨髓基质干细胞表面CD44抗原表达阳性。 介导绿 色荧光蛋白转染新西兰大白兔的骨髓基质干细胞,转染率可达30%左右。 结论:骨髓基质干细胞容易获取、来 源充足、可以大量扩增,可作为骨组织工程的种子细胞。 GFP基因的导入不会影响骨髓间充质干细胞的活性和 生物学特性。
Abstract:
Objective:Toobservetheexpressionof bonemarrowmesenchymal stemcells(BMSCs) invitro,whichis carriedby plasmidandtransfectedby greenfluorescent protein(GFP). Methods: Withplasmidvirus as thecarrier andPT67cells as thepackagingcells,mediatingGFPtotrasfect the NewZealand rabbits. Cells were observed under the phase contrast microscope and fluorescence microscope. GFPexpressionefficiencywas detectedbyflowcytometryinstrument. Results:BMSCs were colonial growthandevenamplificatedwell incontinuous passage culture. Additionally,CD44 onthe surface of BMSCs positively expressed after 2 weeks cultured in vitro. Detected by flowcytometry instrument,the transfection efficiency of GFP was above 30%. Conclusion:BMSCs can be easily obtainedandproliferated,therefore,theymaybeapplicatedas seed-cells inosseous tissueengineering. However,GFPgenewill not affect theactivityandbiological characteristics of BMSCs.

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更新日期/Last Update: 2013-04-20